FTIR (Fourier transform infrared) and GCMS (Gas chromatography-mass spectrometer) analyses were correlated with gravimetric data which reveals that the consortium has removed a wide range of petroleum hydrocarbons in comparison with abiotic control including different aliphatic and aromatic hydrocarbons.Īssam (26.1400° N, 91.7700° E) is the oldest oil producing land from North to East India as oil drilling activity in Asia was first initiated in Digboi, Assam. The consortium showed degradation up to 84.15% of TPH after 5 weeks of incubation, as revealed from gravimetric analysis. cereus R2 (identified by 16s rRNA sequencing) has shown the best result in the desired degradation of crude oil. Consortium 10, which comprises two Bacillus strains namely, Bacillus pumilus KS2 and B. Fourteen different consortia were designed involving both biosurfactant producing and non-producing isolates. Isolates KS2, PG1, and R2 are biosurfactant producers and PG5, R1 are non-producers. From a total of 23 bacterial isolates obtained from three different hydrocarbons contaminated samples five isolates, namely KS2, PG1, PG5, R1, and R2 were selected as efficient crude oil degraders with respect to their growth on crude oil enriched samples.
The purpose of the study was to isolate TPH degrading bacterial strains from various petroleum contaminated soil of Assam, India and develop a robust bacterial consortium for bioremediation of crude oil of this native land. In the present study, the biodegradation capacities of native bacterial consortia toward total petroleum hydrocarbons (TPH) with special emphasis to poly aromatic hydrocarbons were determined. Among others, one of the most favorable methods that can enhance the effectiveness of bioremediation of hydrocarbon-contaminated environment is the application of biosurfactant producing microbes.
The intrinsic biodegradability of hydrocarbons and the distribution of proficient degrading microorganisms in the environment are very crucial for the implementation of bioremediation practices. 2Department of Biotechnology, Gauhati University, Guwahati, India.1Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, India.Kaustuvmani Patowary 1, Rupshikha Patowary 1, Mohan C.
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